Mini-PROTEAN II Electrophoresis Cell Instruction Manual Bio-Rad also recommends that all Mini-PROTEAN II cell components and accessories be cleaned with a suitable laboratory cleaner (such as Bio-Rad Cleaning Concentrate, catalog number 161-0722) and rinsed thoroughly with distilled water before use. WARNING: Do not use alcohol to clean the plastic parts of the Mini-PROTEAN II cell. Stain-free detection as loading control alternative to ... Female Wistar rats and Zucker obese and lean control rats were used in this study, in addition to Caco-2 cells cultured in a standard fashion. Liver samples and Caco-2 cells were homogenized in RIPA buffer and processed for Western blot . We ran a series of protein blots using TGX Stain-Free gradient precast gels (Bio-Rad). Western Blot Protocols - MilliporeSigma | Life Science Following the neutralization, dilute the antibody/ peptide mixture into the appropriate final working volume and proceed with the protocol for the desired test, and proceed with the Western blot procedure as usual. Example Antibody gives good positive results at 2 µg/mL. Normally nitrocellulose is incubated in a final volume of 10 mLs.
This protocol uses the Bio-Rad blotting system. I. Running the Gel: 1. Put protein to be loaded into a 1.5 ml tube, and add Sample Buffer to reach 1xBoil tubes at 100°C for 5 minutes. Store at –20°C until ready for blotting. 2. In the first lane on each gel, add the protein ladder for molecular weight...
Many commercial manifolds are available, most of them with interchangeable units that provide a choice of dot- and slot-blot geometries (Fig. 2.9.3). In this protocol, the DNA to be transferred is heat-denatured and applied to the membrane in a salt buffer. Instruction Manual - University of Sydney photocopy, recording, or any information storage or retrieval system, without permission in writing from Bio-Rad Laboratories. Bio-Rad reserves the right to modify its products and services at any time. This user guide is subject to change without notice. Although prepared to ensure accuracy, Bio-Rad assumes no liability for errors, or for any ... Western Blotting Protocol - Research | MCDB Western Blotting Protocol Protein Isolation: *Always make fresh working solutions before isolation of proteins 1.) Dilute 50X PI solution to 1X PI in TM buffer keeping the solution on ice 2.) Weigh certain amount of tissues and chop them into small pieces 3.) Keep the tissue on dry ice 4.)
Dot Blot - YouTube
Here we describe DNA slot blot repair assay for quantitative detection of NER activity using DNA damage specific antibodies such as anti-CPD and anti-6-4PP. Briefly, genomic DNA irradiated with UV was isolated from cells, and the genomic DNA was vacuum-transferred to a nitrocellulose membrane using a Bio-Dot SF microfiltration apparatus (Bio-Rad). Western Blot Protocol | Bio-Rad
Dot Blot - YouTube
Western Blot Protocol | Bio-Rad This western blot protocol provides a general procedure for use with the majority of Bio-Rad reagents. In some cases specific recommendations are provided on product datasheets, and these methods should always be used in conjunction with product and batch specific information provided with each vial.
Bio-Rad slot blot apparatus Slot Blot Apparatus, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 45 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
XCell II Blot Module - Thermo Fisher Scientific XCell II™ Blot Module The XCell II™ Blot Module is a simple apparatus designed for blotting of mini-gels and is easily inserted into the XCell SureLock™ Mini-Cell in place of the gel/buffer core assembly. The module has rails to guide the unit into the mini-cell. The XCell II™ Blot Module can be used to perform western, General western blot protocol - Abcam
INTRODUCTION Dot blotting is an important technique that is routinely used in research and diagnostic laboratories. Dot blotting is a simple technique to identify a known protein in a biological sample. The ease and simplicity of the technique makes dot blotting an ideal diagnostic tool. Pullen Lab Protocol for Western Blotting with Bio-Rad… For Bio-Rad tank blotting, see protocol 3b in this series. Materials: • Container with ice.11. Prepare ECL detection reagents as directed by the manufacturer’s protocol (we typically use the Li-Cor. WesternSure kit, and instructions are provided with it). Tips for Optimizing Western Blot Protocols | Biocompare… Bio-Rad’s newest tool in this process is software called ImageLab 4.1, which automates the process of blot normalization in a more intuitive way than other“The MPX protocol offers customers the ability to optimize their Western blot conditions in one experiment and works with all detection formats.”